Muscarinic receptor subtypes in rat pancreatic islets: binding and functional studies
نویسندگان
چکیده
منابع مشابه
Quantitative and functional characterization of muscarinic receptor subtypes in insulin-secreting cell lines and rat pancreatic islets.
Expression of muscarinic receptors in rat islets, RINm5F cells, and INS-1 cells was established by reverse transcriptase-polymerase chain reaction (RT-PCR) and quantified by RNase protection. Both methods indicated that m3 and m1 receptors were expressed approximately equally in the various cellular preparations and to a much greater extent than the m5 subtype. However, the cell lines, especial...
متن کاملStudies with Trifluoperazine in Rat Pancreatic Islets
A B S T R A C T The role of calmodulin in insulin secretion from rat pancreatic islets has been examined by the use of trifluoperazine, an inhibitor of calmodulin-Ca++-directed functions. It was found that 30 ,M trifluoperazine caused 50% inhibition, and 100 ,M, up to 73% inhibition of 16.7 mM glucosestimulated insulin release. 100 ,M trifluoperazine caused a similar inhibition of 10 mM glycera...
متن کاملInsulin oversecretion in MSG-obese rats is related to alterations in cholinergic muscarinic receptor subtypes in pancreatic islets.
BACKGROUND/AIMS Impaired pancreatic beta cell function and insulin secretion/action are a link between obesity and type 2 diabetes, which are worldwide public health burdens. We aimed to characterize the muscarinic acetylcholine receptor (mAChR) M1-M4 subtypes in isolated pancreatic islets from pre-diabetic obese rats that had been treated neonatally with monosodium L-glutamate (MSG). METHODS...
متن کاملBinding and functional properties of hexocyclium and sila-hexocyclium derivatives to muscarinic receptor subtypes.
1. We have compared the binding properties of several hexocyclium and sila-hexocyclium derivatives to muscarinic M1 receptors (in rat brain, human neuroblastoma (NB-OK 1) cells and calf superior cervical ganglia), rat heart M2 receptors, rat pancreas M3 receptors and M4 receptors in rat striatum, with their functional antimuscarinic properties in rabbit vas deferens (M1/M4-like), guinea-pig atr...
متن کاملMuscarinic receptor subtypes in airways.
Muscarinic receptor subtypes in the airways appear to subserve different physiological functions. M1-receptors facilitate neurotransmission through parasympathetic ganglia and enhance cholinergic reflexes, but are also localized to alveolar walls. M2-receptors act as autoreceptors on post-ganglionic cholinergic nerves and inhibit acetylcholine release. There is some evidence that they may be de...
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ژورنال
عنوان ژورنال: European Journal of Pharmacology
سال: 1990
ISSN: 0014-2999
DOI: 10.1016/0014-2999(90)90109-j